The effect of intraperitoneally administered glucagon on the lysosomal enzyme activity in the liver of the mice

The experiment was carried out on 20 Swiss male mice divided into experimental (E, n=10) and control (C, n=10) group. E mice were intraperitoneally injected with glucagon (15 μg/kg b.w.) twice daily for eight days, while mice C with 250 μl 0.9% NaCl/mouse (to exclude the injection effect only). In the lysosomal fraction of the liver the activities of acid phosphatase, β-N-acetyl-hexosaminidase, β-glucuronidase, β-glucosidase, lysosomal arylesterase, lysosomal lipase, leucine aminopeptidase and alanine aminopeptidase were determined. Glucagon caused an increase of activity of all studied enzymes except β-glucuronidase and β-glucosidase, where reduced activities were observed.